Article 33-16

DOI: 10.33075/2220-5861-2018-3-110-114


E.A. Snegin1, O.Yu. Artemchuk2

1,2 Belgorod National Research University, Russia, Belgorod, Pobeda St. 85


Based on the method of alkaline gel electrophoresis of isolated cells (Comet Assay for DNA), the level of DNA destruction in four adventive populations of Roman snail (Helix pomatia L.), inhabiting the city of Belgorod and its environs, was studied.  For the analysis, hepatopancreas tissue in forty individuals of a roman snail was used (ten individuals per group). The images were analyzed on an epifluorescence microscope. The data was processed using a program CometScoreТМ v 1,5. The nuclei were ranked by the four stages of DNA destruction. At least 100 nuclei on each preparation were counted. The degree of DNA damage was assessed using the Kraskel-Wallis criterion (DNA-comet index, IDK). The number of cells in the state of apoptosis was also evaluated.

The data obtained indicate to a reliable increase in the index of DNA-comets in a colony prone to pesticides and vehicle exhaust fumes (Maisky settlement, IDK = 0.305 ± 0.070), that lives in a forest belt between the highway and the wheat field. In addition, this colony has the highest percentage of cells in the state of apoptosis (AP), 6.0 ± 0.5%. In the remaining groups, these indicators were smaller (“Donets”: IDK = 0.036 ± 0.020, AP = 1.0 ± 0.3%, “Shopino”: IDK = 0.126 ± 0.056, AP = 0.2 ± 0.1%; “Belgorod”: IDK = 0.136 ± 0.031, AP = 4.9 ± 0.5%).

It is suggested that the accumulation of genotoxic components in a “Maisky” biotope causes a disturbance of cytogenetic stability, disruptions in the work of repair processes in the cells of snails living here, and an increase in gaps in the DNA chain. However, the fact of the presence of this adventitious species in the given territory, as in other parts of Belgorod, testifies to the stability of the genetic apparatus of the roman snail to the action of pollutants of the urbanized environment and creates prerequisites for the further development of the invasive process. We believe that the revealed tendency can serve as a starting point for further monitoring of the investigated snail groups, including both cytogenetic and demographic data. In addition, the obtained result will allow using the method of alkaline gel electrophoresis in bioindication purposes, where not only representatives of the malacofauna, but also other species of animals.

Full text in PDF (RUS)



  1. Ostling O., Johanson K.J. Microelectrophoretic study of radiation-induced DNA damage in individual mammalian cells // Biochemical and Biophysical Research Communications. 1984. Vol. 123, №. 1. P.291–298.
  2. Середенин С.Б., Дурнев А.Д. Фармакологическая защита генома. М.: ВИНИТИ., 1992. 161 с.
  3. Сорочинская У.Б., Михайленко В.М. Применение метода ДНК-комет для оценки повреждений ДНК, вызванных различными агентами окружающей среды // Онкология. 2008. Т. 10, № 3. С. 303–309.
  4. Тронов В.А., Пелевина И.И. Метод ДНК-комет индивидуальных клеток. Принцип и применения метода // Цитология. 1996. Т. 38. № 4/5. С. 71–75.
  5. Снегин Э.А., Ненашева Е.С., Артемчук О.Ю. Оценка степени повреждения ДНК в популяциях наземных моллюсков урбанизированных ландшафтов Среднерусской возвышенности // Научные ведомости БелГУ. Серия Естественные науки. 2011. Вып. 17, № 21 (116). С. 26–30.
  6. Снегин Э.А. Оценка цитогенетической стабильности в природных популяциях наземных моллюсков (на основе метода ДНК-комет) // Онтогенез. 2014. Т. 45, № 3. С. 180–186.
  7. Снегин Э.А., Артемчук О.Ю. Морфогенетический анализ популяций Helix pomatia L. (Pulmonata, Helicidae) юго-восточной и восточной части современного ареала // Экологическая генетика. 2014. Т. XII, № 4. С. 25–37.
  8. Struwe M., Greulich K. O., Suter W. et al. The photo comet assay-A fast screening assay for the determination of photogenotoxicity in vitro // Mutation Research. 2007. Vol. 632, Iss. 1-2. Р. 44–57.

If you have found a spelling error, please, notify us by selecting that text and pressing Ctrl+Enter.

Translate »

Spelling error report

The following text will be sent to our editors: