S.А. Sholar, M.E. Lee

 Marine Hydrophysical Institute of RAS, Russian Federation, Sevastopol, Каpitanskaya Str. 2

DOI: 10.33075/2220-5861-2018-4-17-26

UDC 681.3.06; 535.8; 557.583; 578.4(262.5)


     Optical methods are widely used in monitoring systems of various water areas due to their inherent ability to obtain information about the state of the aquatic environment. Data obtained in the course of optical studies are used by hydrologists, hydrobiologists and other specialists whose interests are related to sea optics. The use of optical express methods allows obtaining information on the required characteristics of water, hydrobionts, soluble organic matter, etc., in situ in real time, without sampling and preparing.

     The purpose of this work is to conduct literature search on the problem of using contact optical methods in studying single-celled aquatic organisms, as well as their parasites – aquatic viruses – with the establishment of the possibility of viral lysis influence on certain physical parameters of their habitat.

     The main optical methods of monitoring the state of aquatic environment were considered. In the course of the literature review, it was found that contact optical methods are actively used to study the distribution of phytoplankton biomass, the photosynthetic activity of radiation, and when analyzing the composition of water with contaminants in the form of dissolved organic and suspended matter.

     However, to study the effect of viral lysis on the optical properties of water as the habitat of aquatic viruses (nano sized hydrobionts), contact optical methods, according to the literature, are practically not used. During the literary search, theoretical evidence was confirmed for the possibility of using contact optical methods for studying the hydrosphere viruses and their role in the functioning of biological and ecological systems of hydrosphere. And the first experiments aimed at studing the effect of viral lysis on the transparency of marine water, performed in 2018, simulating the flowering of phytoplankton and the peak of algal viruses abundance found out the role of viral lysis in increasing the transparency of their habitat – marine water. On the basis of the published literature review and taking into account the first practical results with one of the authors participating in the study, it was suggested that the use of contact optical methods in – aquatic (marine) virology – will provide new facts about the participation of viruses in the functioning of biosystems of reservoirs and about their influence on some physical parameters of their habitat.

Keywords: contact optical methods, multi-spectral light beam attenuation meter, sea optics, sea viruses, photosynthetic activity, fluorescence, light beam attenuation coefficient (BAC).

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  1. Kudryavtsev I. V., Haidukov S. V., Zurochka A.V., Chereshnev V. A. Flow cytometry in experimental biology. Yekaterinburg: RIO Uro RAS, 2012. P. 192.
  2. Li M. E., Shibanov E. B., Martynov O. V. Measurements of spectral properties of the vertical distribution of horizontal irradiance / / Modern problems of optics of natural waters. Moscow, 2015. P. 271-277.
  3. Vinberg G. G. Productivity and protection of marine and fresh water bodies. Moscow: Nauka, 1989. P. 135.
  4. Sirenko L. A., Sakevich A. I., Osipovich L. F. Methods of physiological and biochemical research of algae in hydrobiological practice. Kiev: Naukova Dumka, 1975. P. 247.
  5. Lee M.I., Lee R.I., Martynov O.V. Determina’ tion of bio-optical properties of waters by measuring the spectral characteristics of fluorescence and light scattering in the marine environment // Monitoring systems of environment. Sevastopol: INTS. 2014. issue 20. P. 74–83.
  6. Vavilova V. V., Chernyavsky E. B. On phytoplankton spots in the sea / / Commercial Oceanology. 1977. No. 3. P. 1-18.
  7. Lorenzen C.J. A method for the continuous measurement of the in vivo Chlorophyll concentration // Deep-Sea Research. 1966. Vol. 13. Р. 223–227.
  8. Cullen J.J. The deep Chlorophyll maximum: Comparing vertical profiles of Chlorophyll // Can. J. Fish. Aquat. Res. 1982. Vol. 39. Р. 791–803.
  9. Dickey T. The emergence of concurrent high-resolution physical and bio-optical measurements in the upper ocean // Rev. Geophys. 1991. Vol. 29. P. 383–413.
  10. Behrenfeld M.J., Westberry T.K., Boss E.S. et al. Satellite-detected fluorescence reveals global physiology of ocean phytoplankton // Biogeosciences. 2009. Vol. 6. Р. 779–794.
  11. Gower J.F.R., Brown L., Borstad G.A. Observation of chlorophyll fluorescence in west coast waters of Canada using the MODIS satellite sensor // Can. J. Remote Sens. 2004. Vol. 30. Р. 17–25.
  12. Twardowski M.S., Boss E., Macdonald J.B., Pegau W.S., Barnard A.H., Zaneveld J.R.V. A model for estimating bulk refractive index from the optical backscattering ratio and the implications for understanding particle composition in case I and case II waters // J. Geophys. Res. 2001. Vol. 106. P. 14 129–14 142.
  13. Li M. E., Martynov O. V., Latushkin A. A. Device for determining the content of suspended matter and dissolved organic matter in sea water by measuring the index of light attenuation from the near UV to the red region of the spectrum / / Integrated monitoring system of the Black and Azov seas: mezhdunar. science. Conf.  (Sevastopol, MHI NAS of Ukraine, 24-27 September 2013). 2013. P. 31-35.
  14. Jerlov N.G. Optical Measurements in the Eastern North Atlantic // Med. Oceanogr. Inst. 1961. Vol. 30. P. 1–40.
  15. Ochakovsky Y. E. On the dependence of the total attenuation coefficient upon suspensions in the sea // US Dept. Commerce, Joint Publ. Res. Serv. Rep. 1966. Vol. 36. № 816. P. 16–
  16. Jaksonvil J. M. Transmissometer Manual. Oregon: Sea Tech. Inc. Corvallis. 1989. 22 p.
  17. Kroebel W. The use of optical attenuance meters for biological measurements // Oceans ’77 Conference Record. 1977. P. 534.
  18. Kenneth J. Voss. A spectral model of the beam attenuation coefficient in the ocean and coastal areas // Limnol. Oceanogr. 1992. 37 № 3. P. 501–509.
  19. Levashov D. E., Levashova S. S. The First large-scale bio-optical survey in the South-Eastern part of the Pacific ocean. 2010. Vol. 11. No. 4 (44). P. 653-663.
  20. Mankovskaya E. V., Mankovsky V. I. Information technology for calculating the spectral contribution of components of the marine environment to the indicator of light attenuation for the waters of the Black sea / / environmental control Systems. 2007. Pp. 79-82.
  21. Barth H., Reuter R., Schröder M. Measurement and simulation of substance specific contributions of phytoplankton, gelbstoff, and ‘ mineral particles to the underwater light field in coastal waters // EARSeL eProc. 2000. Vol. 1. P. 165.
  22. Patent 5424840 USA, MPK G01N 21/85; In situ chlorophyl absorption meter / Moore, J.R.V. Zaneveld (USA); заявитель The State of Oregon Acting by and through the State Board of Higher Education on Behalf of Oregon State University (USA). № 285486; заявл. 03.08.1994; опубл. 13.06.1995.
  23. Korchemkina E.N., Latushkin A.A., Lee M.E. Determination of particles concentration in Black Sea waters from spectral beam attenuation coefficient // Proceedings Vol. 10466, 23rd International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics, 30 November 2017 г., Irkutsk, 2007. P. 23–
  24. Yentsch C.S. Measurement of visible light absorption by particulate matter in the ocean // Limnol. Oceanogr. 1962. Vol. 7. 207–217.
  25. Suttle C.A. Marine viruses – major players in the global ecosystem // Nature Reviews Microbiology. 2007. № 5. P. 801–812.
  26. Stepanova O. A. Ecology of allochthonous and autochthonous viruses of the Black sea. Sevastopol: MIR “EXPRESS PRINT”, 2004. P. 308.
  27. Stepanova O. A., Gaisky P. V., Sholar S. A. Influence of viral lysis on some physical parameters of sea water under experimental conditions // Monitoring systems of environment. Sevastopol: INTS. 2018. Vol. 13 (33). Pp. 19-28.
  28. Stepanova O.A., Osipov V.A., Matorin D.M. Use of a method of fluorescence at study of process of interaction between algae virus and sensitive algae culture // Abstracts V Intern. conf. “Bioresourses and Viruses” September 10–13 2007, Kyiv, Ukraine, Kyiv: Phithosociocenter. 94.
  29. Stepanova O.A. Search, isolation and study of Black sea algal viruses 2002–2013. New facts and hypotheses. [Saarbrücken]: Lambert Academic Publishing, 2014. 56 p.
  30. Shpolsky E. V. Application of light scattering to determine molecular weight // Successes of physical Sciences. 1947. Vol. 31. No. 3. P. 417-420.
  31. Oster G. Molecular weights and other properties of viruses as determined by light absorption // Science. 1946. Vol. 103. № 2671. P. 306.
  32. Kubryakov A. A., Stanichny S. V., Kubryakova E. A. Variability of bio-optical characteristics of the Black sea based on measurements of Bio-Argo buoys and satellite data / / Integrated research of the World ocean: II all-Russian scientific conference. Conf. young scientists (Moscow, Institute of Oceanology. P. p. Shirshov Russian Academy of Sciences, April 10-14, 2017). 2017. P. 130-131.
  33. Wommack K.E., Colwell R.R. Virioplankton: Viruses in aquatic ecosys­ tems // Microbiol. and Molec. Biol. Re­ views. 2000. Vol. 64. № 1. P. 69–114.
  34. Ormerod M.G., Tribukait B., Giaretti W. Consensus report of the task force on standardisation of DNA flow cytometry in clinical pathology // Analytical Cellular Pathology. 1998. Vol. № 2. P. 103–110.
  35. Kudryavtsev I. V., Haidukov S. V., Zurochka A.V., Chereshnev V. A. Flow cytometry in experimental biology. Yekaterinburg: RIO Uro RAS, 2012. P. 192.

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