Study of paramagnetic properties of total lipids of marine organisms

 Yu. A. Uss1, B.N. Krasheninnikov2, V.T. Minligareev3

 1Sevastopol Branch of Lomonosov Moscow State University, Russia, Sevastopol, Heroes of Sevastopol st., 7


2 Institute of Natural and Technical Systems, Russia, Sevastopol, Lenin st., 28


3 E. K. Fedorov Institute of Applied Geophysics, Russia, Moscow, Rostokinskaya st., 9


DOI: 10.33075/2220-5861-2018-4-138-142

UDK 577.352.3


     In this work, we present the results of further study of paramagnetic centers, previously identified by the EPR (electron paramagnetic resonance) method  in total lipid extracts from the tissues of Mytilus galloprovincialis L. (gonads) and Squalus achantias (liver).

     The aim of this work was to test the effect of new procedure of extraction of total lipids on the presence and parameters of the EPR spectrum.

     Tissues of marine hydrobionts under study were frozen in the freezer at -200С  for 2 hours before extraction. After thawing, the tissues were homogenized in a porcelain mortar to a creamy state and filled with a mixture of purified hexane and isopropanol (2: 1).Tissue / extraction ratio (g / ml) 1:10. This tissue-extractant ratio was optimal, and the extraction completeness was 97-98%. The extraction was carried out in 100 ml glass centrifuge tubes. Clarification of the extract and washing twice with distilled water in a ratio of 1: 2 (lipid extract: water) was carried out by centrifugation at 3000 rpm at the temperature of +4°C. The washed lipid extract in hexane was evaporated with a rotary evaporator in a vacuum at the temperature of +30°C and re-dissolved in hexane.

    When oxidizing lipid extracts of Mytilus galloprovincialis L. (gonads) and Squalus achantias with lead dioxide in toluene, identical well-resolved EPR spectra were recorded.

   The following conclusions have been made:

– the described method of extraction of total lipids can serve as a basis for developing an optimal scheme for isolation of the active substance with preservation of its antioxidant activity for studying the molecular structure and functional role in cellular structures;

– the probable model of the natural stable free radical redox-fragment is a heterocycle with two closely spaced nitrogen atoms (for example, as in pyrimidine), which are connected by resonance conductivity.

Keywords: one-electron transport, free radicals, lipid peroxidation, lipids of marine hydrobionts, EPR spectroscopy, stable free radical.

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