Biophysical and biochemical techniques in monitoring the coastal waters of Sevastopol (Black Sea)

O.V. Mashukova1, E.N. Skuratovskaya1, J.B. Shilova2

1The A.O. Institute of Marine Biological Research of RAS (IMBR) Russian Federation, Sevastopol, Nakhimov Av., 2


2State Budgetary Educational Institution of Additional Education of the city of Sevastopol “Junior Academy of Sciences of the city of Sevastopol”, Russian Federation, Sevastopol, 163 General Ostryakov Ave.


DOI: 10.33075/2220-5861-2019-1-55-62

UDC 504.064.3:57.08(262.5)   


   Some examples of application of modern biophysical and biochemical techniques in monitoring the coastal waters of Sevastopol are given in the article. Bioluminescence analysis was used as the main biophysical method. The results of spectral analysis of the data obtained by towing a probe SALPA-M prove the existence of a reliable link between the variability of the bioluminescent field and the ecological state of the region under study. Distribution cycling of the bioluminescent field (the area of its variability) can be considered as an indicator of the condition of the bioluminescence field producers.

   The results of many years of research of the coastal waters of Sevastopol demonstrated the usefulness and feasibility of using biochemical methods for estimating the state of ground fish and their habitat. The nature of cause- effect relationship between biochemical parameters and the level of water pollution was determined.

   The material presented indicates the fact that the application of biophysical and biochemical techniques for ecological monitoring of the coastal waters is highly important and rapidly developing direction in modern hydrobiology. Biophysical methods are more indicative when studying the state and productivity of plankton communities of various size structures. Bioluminescence is an essential element of plankton community functioning and can be implemented for a rapid assessment of its structure and functional state. Biochemical techniques enable to observe failures at the earliest stage of damage. Their application for ecological monitoring of the coastal waters is appropriate and worthwhile when studying benthic organisms.

Keywords: ecological monitoring, bioluminescence, bio-indication, biochemical markers, marine biota.

Full text in PDF (RUS)


  1. Rubin A. B. Biophysical methods in environmental monitoring // Soros educational journal. 2000. Vol. 6. No. 4. Pp. 7-13.
  2. Biological methods of water quality assessment: part 1. Bioindication / T. I. Moiseenko, S. N. Gashev, A. G. Selyukov [et al.] / / Vestnik TSU. 2010. no. 7. P. 20-40.
  3. Ecotoxicological studies of the coastal black sea ichthyofauna in the Sevastopol region (ed.: Rudneva I. I.) / A.V. Zavyalov, I. N. Zalevskaya, T. B. Kovyrshina [et al.]. Moscow: GEOS. 2016. P. 360.
  4. Adams S.M. Assessing cause and effect of multiple stressors on marine systems // Mar. Pollut. Bull. 2005. Vol. 51. № 8–12. P. 649–657.
  5. Advances in Marine Biology. Biomarker response of Black Sea scorpion fish Scorpaena porcus to anthropogenic impact (Editors: Adam Kovács and Patrik Nagy) / I.I. Rudneva, E.N. Skuratovskaya, I.I. Chesnokova [et al.] // In Nova Science Publishers. 2016. Chapter 5. Vol. 1. 119–165.
  6. Tokarev Yu. N. Fundamentals of biophysical ecology of hydrobionts. Sevastopol: EKOSI-Hydrophysics. 2006. P. 342.
  7. Tokarev Yu. N., Evstigneev P.V., Mashukova O.V. Planktonic bioluminescents of the World ocean: species diversity, characteristics of light emission in normal and anthropogenic conditions. Sevastopol: Orianda. 2016. 340 p.
  8. Using chlorophyll fluorescence to assess the production characteristics of phytoplankton / D. N. Matorin, S. I. Pogosyan, V. A. Osipov [et al.] / / Biotechnology, ecology, environmental protection / ed. by A. P. Sadchikov, S. V. Kotelevtseva. M.: publishing house “grafikon-print”. 2005. P. 189–194.
  9. Assessment of aquatic organisms biochemical status / N. N. Nemova, O. V. Meshcheryakova, L. A. Lysenko [and others] // Transactions of Karelian research centre. 2014. No. 5. P. 18-29.
  10. Lukyanova O. N., Korchagin V. P. Integral biochemical index of the state of aquatic organisms under pollution conditions // Proceedings of the Russian Academy of Sciences. Biological series. 2017. No. 2. P. 174–180.
  11. The bioluminescence field as an in-dicator of the spatial structure and physiological state of the planktonic community at the Mediterranean sea basin / Yu.N. Tokarev, E.P. Bityukov, R. Williams [et al.] // The eastern Mediterranean as a laboratory basin for the assessment of contrasting ecosystems. The Netherlands: Kluwer Academic Publishers. 1999. P. 407–416.
  12. Aronsohn M.D. Studying Bioluminescent Blooms in the Arabian Sea // State of planet. December 4, 2017 URL:
  13. Identification of spatial heterogeneity of the bioluminescence field using the towed hydrobiophysical complex “Salpa-M” / Yu. N. Tokarev, V. I. Vasilenko, V. F. Zhuk [et al.] / / Marine environmental journal. 2014. Vol. 13. No. 2. P. 71-81.
  14. Skuratovskaya E. N. State of the antioxidant system of the blood of black sea fish in the conditions of complex chronic pollution: author’s abstract … Cand. Biol. Sciences. Odessa. 2009. P. 20.
  15. Comparative analysis of biochemical parameters of the sea ruff Scorpaena porcus L. from the bays of Sevastopol with different levels of pollution / E. N. Skuratovskaya, T. B. Kovyrshina, I. I. Rudneva [et al.] / / Marine biological research: achievements and prospects: collection of materials vseros. scientific-practical Conf. with Intern. participation dedicated to the 145th anniversary of the Sevastopol biological station (Sevastopol, September 19-24, 2016). Sevastopol: EKOSI-Hydrophysics. 2016. Vol. 3. P. 222-225.
  16. Application fish blood biomarkers in evaluation of marine environment health / I.I. Rudneva, E.N. Skuratovskaya, I.I. Dorohova [et al.] // World Journal of Science and Technology. 2012. Vol. 2. № 7. Р. 19–25.
  17. Organochlorine compounds in the black sea yershe Scorpaena porcus LINNAEUS, 1758 in the water area of Sevastopol: spatial distribution and biological response to the level of accumulation of pollutants / L. V. Malakhova, E. N. Skuratovskaya, T. V. Malakhova [et al.] / / Marine biological journal. 2018. Vol. 3.4. P. 51-63.
  18. Lukyanenko V. I., Khabarov M. V. Albumin system of blood serum of different sturgeon species in ecology. Yaroslavl: VVO REA. P. 205.
  19. Fish bioaccumulation and biomarkers in environmental risk assessment: A review / van der Oost, J. Beyer, N.P.E. Vermeulen // Environ. Toxicol. and Pharmacol. 2003. V. 13. P. 57–149.
  20. Kovyrshina T. B. Application of biomarkers of the round goby Neogobius melanostomus (Pallas, 1814) to assess the ecological state of the coastal waters of the Black and Azov seas: author’s abstract … Cand. Biol. Sciences. Sevastopol. 2017. P. 22.
  21. Bioindication of the Environmental State of Marine Areas with the Use of Fish Biomarkers / I.I. Rudneva., E.N. Skuratovskaya, I.I. Dorokhova, Yu.A. Grab // Water resourses. Vol. 38, iss. 1. Р.107–112.
  22. Shilova Yu. B. Indicators of protein metabolism as status indicators of black sea fishes in different habitat conditions // Novoe Vremya: materials of the XIV Intern. salon of inventions and technologies (Sevastopol, September 27-29, 2018). 2018. P. 323-325.