Methodical approaches to search, isolation and study of Black Sea algal viruses

O.A. Stepanova1, P.V. Gaisky1, S.A. Sholar2

1Institute of Natural and Technical Systems, Russian Federation, Sevastopol, Lenin St., 28

E-mail: solar-ua@ya.ru

2Marine Hydrophysical Institute of RAS, Russian Federation, Sevastopol, Kapitanskaya St., 2

 E-mail: sa.sholar@mail.ru

DOI: 10.33075/2220-5861-2019-3-102-111

UDC 578.4 (262.5); 681.3.06; 535.8

Abstract:

   Taking into account the relevance of the problems of a new scientific direction in hydrobiology – marine (aquatic) virology and its insufficiently active development in our country, the purpose of the article is to provide brief information on some methodological approaches when searching, isolating and studying of Black Sea algal viruses, including at the level of model experiments.

   The review of the available literature with a partial description of the ways and methods for searching, isolating and studying of Black Sea algal viruses, conducted since 2002, shows that in the course of the research, in addition to classical approaches some methods improved and / or developed by the authors were used. This made it possible to identify and describe algal viruses that are new for the science or new for the Black Sea ecosystem, as well as expand knowledge of the ecology of marine viruses.

   In a number of cases to obtaining new knowledges contributed the use of the firstly studied material (clinical material and material from hydrobionts), as well as model experiments, during which a unique laboratory installation and experimental laboratory stand were developed. Based on the results of the search and isolation of algal viruses from the Black Sea samples and their analysis, it is proposed to use algal viruses monitoring as an environmental indicator and as a component of environmental monitoring, and algal viruses as biological indicators and / or markers.

   The results of the experimental study of viral lysis using the created laboratory installation and the test laboratory stand made it possible to record facts that are new to sea physics and optics at the level of biophysical processes of contact between the virus and the host with using of Black Sea micro biota.

Keywords: methods for studying the biology and ecology of algal viruses, microalgae, Black Sea, universal laboratory installation, laboratory stand, viral lysis in marine physics and optics.

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