ESTIMATION OF RED AUTO-FLUORESCENCE OF CHLOROPHYL а IN DINOFLAGELLATES BY LUMINESCENT MICROSCOPY

L.V. Stelmakh, I.M. Mansurova

 A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, RF, Sevastopol, Nakhimov Av., 2

E-mail:  lustelm@mail.ruira.mansurova2013@yandex.ua

DOI: 10.33075/2220-5861-2019-4-128-134

Abstract:

     The aim of this work was to substantiate a methodological approach to the estimation of chlorophyll a red autofluorescence in dinoflagellates on the example of the Black Sea species Prorocentrum micans.

     The object of the study was an algologically pure microalgae culture Prorocentrum micans Ehrenberg (Dinophyta), isolated from plankton of the Black Sea and maintained in the collection of the Department of Ecological Physiology of Algae of the A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS.

     An approach to studying red autofluorescence of chlorophyll a in dinoflagellates using green light to excite this process was proposed. Applying green light for dinoflagellates was possible due to the presence of peridinin in their light-absorbing antennas. In this group of algae, it is the main light-absorbing pigment that transfers absorbed light energy to chlorophyll a. It provides intensification of the autofluorescence of chlorophyll and permits its estimation in individual algal cells. Using the proposed approach, a high degree of heterogeneity of the functional state of individual cells of dinoflagellate P. micans in the stationary phase of their growth was revealed. In the logarithmic growth phase, the functional activity of individual cells of the studied algae species varied within rather narrow limits. For the entire artificial algae population, the average values of the intensity of red autofluorescence of chlorophyll a, the efficiency of photosystem 2, and the specific content of chlorophyll a per cell decreased with the growth of the accumulative culture and its transition from a logarithmic to a stationary growth phase.

Keywords: dinoflagellates, autofluorescence of chlorophyll a, luminescent microscopy.

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