L.V. Stelmakh, I.M. Mansurova
A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, RF, Sevastopol, Nakhimov Av., 2
The aim of this work was to substantiate a methodological approach to the estimation of chlorophyll a red autofluorescence in dinoflagellates on the example of the Black Sea species Prorocentrum micans.
The object of the study was an algologically pure microalgae culture Prorocentrum micans Ehrenberg (Dinophyta), isolated from plankton of the Black Sea and maintained in the collection of the Department of Ecological Physiology of Algae of the A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS.
An approach to studying red autofluorescence of chlorophyll a in dinoflagellates using green light to excite this process was proposed. Applying green light for dinoflagellates was possible due to the presence of peridinin in their light-absorbing antennas. In this group of algae, it is the main light-absorbing pigment that transfers absorbed light energy to chlorophyll a. It provides intensification of the autofluorescence of chlorophyll and permits its estimation in individual algal cells. Using the proposed approach, a high degree of heterogeneity of the functional state of individual cells of dinoflagellate P. micans in the stationary phase of their growth was revealed. In the logarithmic growth phase, the functional activity of individual cells of the studied algae species varied within rather narrow limits. For the entire artificial algae population, the average values of the intensity of red autofluorescence of chlorophyll a, the efficiency of photosystem 2, and the specific content of chlorophyll a per cell decreased with the growth of the accumulative culture and its transition from a logarithmic to a stationary growth phase.
Keywords: dinoflagellates, autofluorescence of chlorophyll a, luminescent microscopy.
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[IEEE] L.V. Stelmakh and I. M. Mansurova, “ESTIMATION OF RED AUTO-FLUORESCENCE OF CHLOROPHYL а IN DINOFLAGELLATES BY LUMINESCENT MICROSCOPY,” Monitoring systems of environment, vol. 4, pp. 128–134, Dec. 2019.
LIST OF REFERENCES
- Mieszkowska N. Multinational, integrated approaches to forecasting and managing the impacts of climate change on intertidal species / N. Mieszkowska, L. Benedetti-Cecchi, M.T. Burrows [et al.] // Marine Ecology Progress Series, 2019. Vol. 613. P. 247–252.
- L.V. Stelmakh, E.A. Kuftarkova, A.I. Akimov [et al. ] /Using variable chlorophyll fluorescence in vivo to assess the functional state of phytoplankton // Monitoring systems of environment. 2010. Vol. 13. P. 263–268.
- Mansurova I.M. The Influence of light on the specific growth rate Zinovyevich algae of the Black sea // Marine ecological journal. 2013. Vol. 12. No. 4. P. 73-78.
- Tsilinsky V.S., Suslin V.V., Finenko Z.Z. Seasonal dynamics of the efficiency of the photosynthetic apparatus of phytoplankton in the coastal areas of the Black sea / / Oceanology. 2018. Vol. 58. No. 4. P. 593-600.
- Stelmakh L., Gorbunova T. Emiliania huxleyi blooms in the Black Sea: influence of abiotic and biotic factors // Botanica, 2018. Vol. 24, No. 2. P. 172–184.
- Geider R.J. Light and temperature dependence of the carbon to chlorophyll ratio in microalgae and cyanobacteria: implications for physiology and growth of phytoplankton // New Phytologist, 1987. Vol. 106. P. 1–34.
- Aldridge D., Purdie D.A., Zubkov M.V. Growth and survival of Neoceratium hexacanthum and Neoceratium candelabrum under simulated nutrient-depleted conditions // Journal of Plankton Research, 2013. Vol. 36, No. 2. P. 439–449.
- Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS Report Nr. 19, vi+170 pp. Reprint of the IOC Manuals and Guides No. 29, UNESCO 1994 / Knap, A., Michaels, A., Close, A., Ducklow, H., Dickson, A. (eds.). 1996.
- S.I. Pogosyan, S.V. Galchuk, Yu.V. Kazimirko [et al. ] /Application of the “Mega-25” fluorimeter for determining the amount of phytoplankton and evaluating the state of its photosynthetic apparatus // Water: chemistry and ecology. 2009. No. 6 (12). P. 34-40.
- Litvinyuk D.A. Marine zooplankton and methodological problems of its study. Abstract. dis. Cand. Biol. sciences’. Moscow. 2015. P.27.
- Geider R.J., MacIntyre, H.L., Kana, T.M. Dynamic model of phytoplankton growth and acclimation: responses of the balanced growth rate and the chlorophyll a : carbon ratio to light, nutrient limitation and temperature // Marine Ecology Progress Series, 1997. Vol. 148. P. 187–200.
- Stelmakh L.V. Functional state and some structural characteristics of marine plankton algae at different levels of nutrient availability. Questions of modern Algology. 2018. No. 1 (16). URL: http://algology.ru/1252
- Polívka T., Hiller R.G., Frank H.A. Spectroscopy of the peridinin-chlorophyll-a protein: insight into light-harvesting strategy of marine algae // Archives of biochemistry and biophysics, 2007. Vol. 458, No. 2. P. 111–120.
- The Unique Photophysical Properties of the Peridinin-Chlorophyll-a-Protein / Carbonera, D., Di Valentin, M., Spezia, R. [et al.] // Current Protein and Peptide Science, 2014, Vol. 15, No. 4. P. 332–350.