Study of the toxylogical status of surface waters of the Ash-kadar river using biotesting methods

N.O. Znobishcheva, T.Kh. Galiev, R.R. Khatmullina, I.V. Ovsyannikova, A.A. Islamutdinova

«Ufa State Petroleum Technological University» branch in Sterlitamak, RF, Sterlitamak, October Av., 2.


DOI: 10.33075/2220-5861-2020-2-97-101

UDC 543.97                                                                  


   The work focuses on a toxicological assessment of the surface water of the Ashkadar river using the biotesting method with the seeds of Lepidium sativum “Zabava” variety as a test object. The experiment was carried out according to the Methodology for determining the toxicity of drinking, ground, surface and wastewater,and chemical solutions by measuring germination rate, average length and average weight of dry seedlings of Lepidium sativum seeds PND F T 14.1: 2: 4.19-2013.

   Water sampling from the Ashkadar river was made in February, April and October of 2018. The experiment was carried out in laboratory conditions. 30 seeds of Lepidium sativum were laid out on filter paper into Petri dishes. Then a number of dilutions (1: 2, 1: 4, 1: 8 and 1:16) were prepared from the analyzed samples. Distilled water was used for dilution and as a control one. There were 3 samples of each dilution and control water. 5 ml of undiluted water and a sample with dilutions of 1: 2, 1: 4, 1: 8 and 1:16 were added into each Petri dish with seeds. Seed germination (%), the average length of the seedlings (mm) and the average weight of dry seedlings (mg) were measured on the seventh day after the start of the experiment. Statistical data processing was performed using the Statistica_5.0 program.

   The results of this work allowed concluding that the average seedling length is the most sensitive parameter. The negative impact of anthropogenic activity on the Ashkadar river has also been identified. The obtained results can be used to model the dynamics of the ecological situation in the studied water bodies. Such information will be the forecast component of the environmental monitoring system of water bodies.

Keywords: biotest, Lepidium sativum, toxicity, average length, seed germination, dry seedling weight, correlation coefficient.

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