Changes in infectious titer of Black sea algal virus of the microalgae tetraselmis viridis under the influence of a constant magnetic field

O.A. Stepanova1, P.V. Gaisky1, S.A. Sholar2

1Institute of Natural and Technical Systems, RF, Sevastopol, Lenin St., 28

E-mail: solar-ua@ya.ru

2FRC Marine Hydrophysical Institute of RAS, RF, Sevastopol, Kapitanskaya St., 2

E-mail: sa.sholar@mail.ru

DOI: 10.33075/2220-5861-2020-4-35-39

UDC 577.35. 578.4 (262.5)

Abstract:

   The fact of the influence of electromagnetic and magnetic fields on biological objects, established by the researchers, is also described as a depressing effect on the reproduction of viruses. Currently, there is information about the possibility of using a low-frequency magnetic field to treat infections caused by COVID-19, as well as about the development of a method for destroying the most dangerous potato viruses with a pulsed magnetic field. The aim of the study is to establish the effect of a constant unidirectional magnetic field on the infectious titer (change in concentration) of Black Sea algal viruses.

   The experiments were carried out using one of the strains of the algal virus of the microalgae Tetraselmis viridis (TvV-SI1) in an installation created on the basis of the laboratory of the Institute of Natural and Technical Systems, in the base of which there are two magnetic disks rigidly fixed at a distance of 5 cm and a constant unidirectional magnetic field with a magnetic induction 600 Gauss (G) is created between them. Glass tubes with a viral suspension were placed between the disks in the magnetic field (experiment). The titer of viruses was determined before and after exposure to a magnetic field using a patented author’s method.

   In three experiments with duration of 24 and 36 h, it was found that with a low initial titer of the algal virus strain TvV-SI1, its stay in a magnetic field for 24 h led to an increase in the infectious titer by one order of magnitude. However, when the virus with a higher infectious titer was in a magnetic field for 24 h, no changes in the titer were observed, and after 36 h, its decrease by one order of magnitude was recorded. The results obtained indicate the need for further research in this direction, incl. using a wider range of algal viruses.

Keywords: algal viruses, vital infectious titer, microalga Tetraselmis viridis, constant unidirectional
magnetic field.

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