Biomarkers of behavioral reactions of mussels in automated biomonitoring systems under conditions of aquatic environment pollution by drill cuttings and oil hydrocarbons

by

V.V. Trusevich, V.Yu. Zhuravskiy, E.V. Vyshkvarkova, K.A. Kuzmin, V.Zh. Mishurov

Institute of Natural and Technical Systems, RF, Sevastopol, Lenin St., 28

E-mail: trusev@list.ru

DOI: 10.33075/2220-5861-2020-4-50-57

UDC 504.064.3:574:681.5 

Abstract: 

   Creating highly efficient automated systems for environmental monitoring of waters is becoming an increasingly urgent need due to rapid expansion of oil hydrocarbon production zones on the sea shelves, accompanied by both chronic and accidental massive releases of highly toxic substances into the ecosystems of these zones. As such a system, for the first time in the Russian Federation, we have proposed a complex of automated biosensor monitoring and early warning, in real time, based on the behavioral responses of bivalve mollusks. Our complex is an analogue of the Musselmonitor monitoring system, aimed at being used in natural conditions of water bodies. To assess the efficiency of the complex functioning in laboratory conditions, the sensitivity of the Black Sea mussel to the impact of one of daily (for 2 hours) sequentially increasing concentrations of aqueous extracts of drill cuttings (25, 50 and 500 mg/L) and diesel fuel (0.01 and 0.021 mg/l.)was studied, which to some extent simulates a normally existing situation in the areas of hydrocarbon exploration and production. It has been established that the proposed complex makes it possible to confidently detect, even in the first minutes, the appearance of toxicants in the aquatic environment in concentrations characteristic of low-toxic zones in the regions of oil and gas production and drilling operations. It is shown that repeated exposure of mollusks to extracts of drill cuttings and diesel fuel, even at low concentrations for several days, caused a general decrease in the amplitude of valve opening and disruption of the diurnal rhythm of mollusks, which is probably the “effect of accumulation of exposure”.

Keywords: Black Sea mussel, behavioral responses, automated biomonitoring, drill cuttings extracts, diesel fuel.

To quote: Trusevich, V.V., V.Yu. Zhuravskiy, E.V. Vyshkvarkova, K.A. Kuzmin, and V.Zh. Mishurov. “Biomarkers of Behavioral Reactions of Mussels in Automated Biomonitoring Systems Under Conditions of Aquatic Environment Pollution by Drill Cuttings and Oil Hydrocarbons.” Monitoring Systems of Environment no. 4 (December 24, 2020): 50–57. doi:10.33075/2220-5861-2020-4-50-57.

Full text in PDF (RUS)

LIST OF REFERENCES

  1. Environmental monitoring of the oil and gas industry / M.N. Saksonov, A.D. Abalakov, L.V. Danko [et al.] // Physicochemical and biological methods: tutorial. Irkutsk: Publishing house Irkutsk. university. 2005.114 p.
  2. Patin S.A. Oil and ecology of the continental shelf. Sec. ed., rev. and add. Moscow: VNIRO Publishing. 2017. 345 p.
  3. Kramer K.J.M., Botterweg M. Aquatic biological early warning systems. Bioindicators and environ-mental management. D.W. Jeffrey, B. Madden (eds). Lon-don: Academic Press. 1991. Р. 95–126.
  4. Borcherding J. Ten years of practical experience with the Dreissena-Monitor, a biological early warning system for continuous water quality monitoring // Hydrobiologia. 2006. Vol. 556. P. 417–426.
  5. Trusevich V.V., Gaiskiy P.V., Kuzmin K.A. Automated biomonitoring of the aquatic environment using the reactions of bivalve mollusks // Marine Hydrophysical Journal. 2010. No. 3. P. 75–83.
  6. Biomarkers of behavioral reactions of the Black Sea mussel for automated biomonitoring of the ecological state of the aquatic environment / V.V. Trusevich, P.V. Gaisky, K.A. Kuzmin [et al.] // Monitoring systems of environment. 2015. No. 1 (21). P. 13-18
  7. Trusevich V.V., Kuzmin K.A., Mishurov V.Zh. Monitoring of the aquatic environment using freshwater bivalve mollusks // Monitoring systems of environment. 2017. No. 7 (27). P. 83–93.
  8. Bakhmet I.N. Characteristic features of adaptation of the edible mussel Myttilus edulis L. to pollutants // Uchenye zapiski Petrazavodskogo gos. un-ta. Biology. 2013. No. 8. P.17–20.
  9. Mussel Mytilus edulis L. White Sea as an indicator under the influence of dissolved oil products / I.N. Bakhmet, N.N. Fokina, Z.A. Nefedova [et al.] // Proceedings of the Karelian Scientific. center of RAS. 2012. No. 2. P. 38–46.
  10. Skidchenko V.S., Vysotskaya R.U., Nemova N.N. The spectrum of acid deoxyribonuclease isoforms in the tissues of the mussel Mytilus edulis under the conditions of model intoxication with petroleum products // Trudy Karelskogo nauch. center of RAS. 2012. No. 2. P.131-138.
  11. Marigómez I., Garmendia L., Soto M., Orbea A., Izagirre U., Cajaraville M.P. Marine ecosystem health status assessment through integrative biomarker indices: a comparative study after the Prestige oil spill «Mussel Watch» // Ecotoxicology. 2013. Vol. 22. P. 486–505.
  12. Frantzen M., Regoli F., Nahrgang J., Ambrose W., Geraudie P., Benedetti M., Locke W., Camus L. Biological effects of mechanically and chemically dispersed oil on the Icelandic Scallop (Chlamys islandica) // Ecotoxicology and environmental safety. 2016. Vol. 127. P. 95–107.
  13. Milinkovitch T., Geraudie P., Ca-mus L., Hélène V.H., Guyon T. Biomarker modulation associated with marine diesel contamination in the Iceland scallop (Chlamys islandica) // Environmental Science Pollution Research. 2015. Vol. 22. P. 19292–19296.
  14. Redmond K.J., Berry M., Pampanin D.M., Andersen O.K. Valve gape behavior of mussels (Mytilus edulis) exposed to dispersed crude oil as an environmental monitoring end-point // Marine Pollution Bulletin. 2017. Vol. 117. P. 330–339.
  15. Data analysis for automation of aquatic environment biomonitoring in the Black Sea region / V.Yu. Zhuravsky, E.N. Voskresenskaya, V.V. Trusevich [et al.] // Monitoring systems of environment. 2019. No. (4) 38. P. 66–71.
  16. Robson A.A., Garcia de Leaniz C., Wilson R.P., Halsey L.G. Behavioural adaptations of mussels to varying levels of food availability and predation risk // Journal of Molluscan Studies. 2010. Vol. 76(4). P. 348–353.
  17. Curtis T.M., Williamson R., De-pledge M.H. Simultaneous, long-term monitoring of valve and cardiac activity in the blue mussel Mytilus edulis exposed to copper // Marine Biology. 2000. Vol. 136. P. 837–846.
  18. Features of valve movement and cardioactivity of bivalve mollusks under the action of various stressors / S.V. Kholodkevich, T.V. Kuznetsova, V.V. Trusevich [et al.] // Journal of Evolutionary Biochemistry and Physiology. 2009. Vol. 45. No. 4. P. 432–434.
  19. Ortmann С., Grieshaber M. Energy metabolism and valve closure behaviour in the Asian clam Corbicula fluminea // Journal of Experimental Biology. 2003. Vol. 206. P. 4167–4178.
  20. De Zwaan A., Wijsman T.C.M. Re-view: Anaerobic metabolism in bivalvia (Mollucsa) // Characteristics of anaerobic metabolism. 1976. Vol. 56B. P. 313–324.
  21. Sandrini-Neto L., Pereira L. da Silva, Martins C.C., de Assis H.C.S., Camus L. Antioxidant responses in estuarine invertebrates exposed to repeated oil spills: Effects of frequency and dosage in a field manipulative experiment // Aquatic Toxicology. 2016. Vol. 177. P. 237–249.
  22. Sukharenko E.V., Nedzvetsky V.S., Kyrychenko S.V. Biomarkers of metabolism disturbance in bivalve molluscs induced by environmental pollution with processed by-products of oil // Biosystems Diversity. 2017. 25 (2). P. 113–118.
  23. Geraudie P., Bakkemo R., Milinkovitch T., Thomas-Guyon H. First evidence of marine diesel effects on biomarker responses in the Icelandic scallops, Chlamys islandica // Environmental Science and Pollution Research. 2016. Vol. 23. Р. 16504–16512.
  24. Gudimov A.V. Biotesting of diesel fuel for operational bioindication // Sb. St. Vseros. scientific. conf. with int. participation, dedicated to the 125th anniversary of prof. V.V. Vodyanitskiy “Pollution of the marine environment: environmental monitoring, bioindication, regulation”. Sevastopol. 2018. P. 78–82.

 

Loading