Аnalisis of the changes in the concentration of ammonium nitrogen in water with the young mussels contained in aeriated non-flowing conditions in food insufficiency

I.I. Kazankova1, M.M. Bayrite2

1Institute of Natural and Technical Systems, RF, Sevastopol, Lenin St., 28

E -mail: ikazani@bk.ru

2The A.O. Kovalevsky Institute of Biology of Southern Seas of RAS, RF, Sevastopol, Nachimov Av., 2

DOI: 10.33075/2220-5861-2020-4-86-92

UDC 574.24:504.064.36 


   Metabolites released during the vital activity of hydrobionts can accumulate in the aquatic environment and negatively affect the state of organisms. These substances include ammonium, the maximum permissible concentration (MPC) of which in the aquatic environment of reservoirs is about 3 mg l-1 for nitrogen. The article deals with changes in the concentration of ammonium in water during an eight-day experiment on the non-flowing content of immature mussels Mytilus galloprovincialis 24–26 mm long in food insufficiency. The remaining conditions of the experiment were favorable – aeration, water temperature 20–21 °C, daytime illumination below 50 lx. The food deficiency leading to starvation of mussels was ensured by the fact that the replacement of water with fresh sea water in the vessels took place every 2–4 days, there was one liter of water per mussel. Previously, the method of non-invasive high-frequency valvometry was determined that under the conditions listed above, mussels have an increase in the total time of the shell in the closed state, which was associated with food deficiency. The effect of metabolites released by mussels on the movement of the valves was not considered. To track the accumulation of ammonium released by mussels in the environment, its concentration was measured at the beginning and end of 2–4-day exposures. Initial concentrations of ammonium were 2.6–5.7, and at the end of exposures – 1.8–20.6 µg·l-1, which is 2–3 orders of magnitude lower than the MPC. Thus, under the conditions of the experiment, at the end of the exposures, compared with its initial content in fresh sea water, both an increase and a decrease in the concentration of ammonium nitrogen was observed. The decrease in the concentration of ammonium is most likely due to the nitrification, the increase is due to the release of waste products, as well as the length of time between their release by shellfish and the moment of taking a water sample for analysis. The obtained data allow us to conclude that there is a low probability of a negative effect of ammonium nitrogen released by mussels on the movement of their valves in the conditions of the experiment. The possibility of influencing the behavioral reactions of mussels of nitrites and nitrates, as products of ammonium nitrification, is to be determined in further studies.

Keywords: juvenile mussel Mytilus galloprovincialis, ammonium nitrogen, food deficiency, Black Sea.

To quote: Kazankova, I.I., and M.M. Bayrite. “Аnalisis of the Changes in the Concentration of Ammonium Nitrogen in Water with the Young Mussels Contained in Aeriated Non-Flowing Conditions in Food Insufficiency.” Monitoring Systems of Environment no. 4 (December 24, 2020): 86–92. doi:10.33075/2220-5861-2020-4-86-92.

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  1. Trusevich V.V., Kuzmin K.A., Mishurov V.Zh. Monitoring of the aquatic environment with the use of freshwater bivalve mollusks // Environmental control systems. 2017. 7(27). P. 83–93.
  2. Gnyubkin V.F. Circadian rhythm of leaf movement in the bivalve mollusk Mytilus galloprovincialis // Biology of the sea. 2010. 36 (6). P. 415–423.
  3. Gaisky P.V., Stepanova O.A. Behavioral reactions of freshwater molluscs Unio pictorum to a number of widespread abiotic chemical pollutants // Environmental control systems. 2020. 2 (40). with non-flowing content of mollusks.
  4. I.I. Kazankova, S.V. Kazantsev, P.V. Gaisky [et al.] / Environmental control systems–2019: theses of dokl. International Scientific and Technical Conf. (Sevastopol, 12–13 Sept., 2019) Sevastopol. 2019. P. 22.
  5. Kazankova I.I., Kazantsev S.V., Shlyk V.A. Features of the movement of the valves of the mussel Mytilus galloprovincialis in the early stages of starvation and monitoring of the aquatic environment / Actual problems of environmental control: proceedings of the seminar (Sevastopol, 10–11 November 2020). Sevastopol: Kulikov A.S., 2020. P. 51.
  6. Biomarkers of behavioral responses of black sea mussels for automated biomonitoring of the ecological status of the aquatic environment / V.V. Trusevich, P.V. Gaisky, K.A. Kuzmin [et al.]. / Environmental control systems. 2015. 1 (21). P. 13–18.
  7. Relationship between filtration activity and food availability in the Mediterranean mussel Mytilus galloprovincialis / O. Maire, J.M. Amouroux, J.C. Duchêne [et al.] / Marine Biology. 2007. V. 152. № 6. P. 1293–1307.
  8. Guide to methods of chemical analysis of marine waters / 1993. St. Petersburg: Hydrometeoizdat. 265 p.
  9. Diagnosis of the current state of the waters of the Sevastopol coast (Black Sea) based on the results of monitoring of hydrochemical characteristics / V.I. Gubanov, Yu.A. Malchenko, E.A. Kuftarkova [et al.]. / Ecological safety of coastal and shelf zones and complex use of shelf resources. ECOSI-Hydrophysics. 2004. № 10. P. 141–148.
  10. Slepchuk K.A., Khmara T.V., Mankovskaya E.V. Comparative assessment of the trophic level of the Sevastopol and Southern bays using the E-TRIX index // Marine Hydrophysical Journal. 2017. №. 5. P. 67–78.
  11. Yasakova O.N. Seasonal dynamics of phytoplankton of the Novorossiysk Bay in 2007 // Marine Ecological Journal. Sevastopol. 2013. 12 (1). P. 92–102.
  12. Finenko G.A. The role of detritus in the diet of the bivalve mollusk Mytilus galloprovincialis Lam. // Ecology of the sea. 1988. Vol. 28. P. 64–69.
  13. Kruk L.S. Investigation of the filtration rate of the Black Sea mussel (Mytilus galloprovincialis) depending on the concentration of food, body weight and water temperature / All-Union Conference on Commercial Invertebrates.Sevastopol, April 1986. Abstracts of reports. M., 1986. Part 2. P. 244–245.
  14. Pechen-Finenko G.A. Water filtration rate of Mytilus galloprovincialis Lam. as a function of body weight and temperature. // Ecology of the sea. 1987. Vol. 25. P. 54–62.
  15. Vyalova O.Yu. Features of energy and nitrogen metabolism of immature Black Sea of mussels Mytilus galloprovincialis Lam. in the conditions of the experiment / Author’s abstract … cand. biol. sciences’. Sevastopol. 2000. 17 p.
  16. The hydrological and hydrochemical regime of the Sevastopol Bay and its changes under the influence of climatic and anthropogenic factors / V.A. Ivanov, E.I. Ovsyany, L.N. Repetin [et al.] / Sevastopol. 2006. 90 p. (Preprint / MGI NASU).
  17. Kondratiev S.I. Features of the distribution of dissolved oxygen in the waters of the Sevastopol Bay in 2006–2007 // Marine Hydrophysical Journal. 2010. № 2. P. 63–76.
  18. List of fishery standards: maximum permissible concentrations (MPC) and approximately safe levels of exposure to harmful substances for water of aquatic objects with fishery significance. 1999. Moscow: VNIRO Publishing House. 304 p.
  19. Ecological assessment of the current state of waters in the area of interaction of the Sevastopol Bay with the adjacent part of the sea / E.A. Kuftarkova, V.I. Gubanov, N.P. Kovrigina [et al.] / Marine Ecological Journal. 2006. 5 (1). P. 72–91.
  20. A comprehensive study of the ecological status of the coastal water area of Sebastopol (Western Crimea, Black sea) / V.I. Ryabushko, S.V. Shchurov, N.P. Kovrigina [et al.] / Ecological safety of coastal and shelf zones of the sea. 2020. № 1. P. 103–118.
  21. Le T.T.H., Fettig J., Meon G. Kinetics and simulation of nitrification at various pH values of a polluted river in the tropics // Ecohydrology & Hydrobiology. 2019. 19 (1). P. 54–65.
  22. Ward B.B., Olson R.J., Perry M.J. Microbial nitrification rates in the primary nitrite maximum off southern California // Deep Sea Research Part A. Oceangraphic. 1982. 29 (2).P. 247–255.
  23. Vasechkina E.F., Kazankova I.I. Individual variability of the rate of physiological processes in bivalves // Environmental safety of the coastal and offshore zones of the sea. 2018. № 1. P. 23–31.