Experimental evaluation of the spectral coefficient of light absorption energy by microalgae cells based on the culture optical characteristics

by

R.P. Trenkenshu, T.M. Novikova, Ya.D. Zhondareva

A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS

RF, Sevastopol, Nakhimov Av., 2

E-mail: novikovat.m@ibss-ras.ru

DOI: 10.33075/2220-5861-2025-3-84-89

UDC 579:582.26/.27:577.1:519.2                             

EDN: https://elibrary.ru/vmnkzo

Abstract:

The paper presents an analysis of the optical properties of microalgae culture. From a physicochemical point of view, microalgae culture is a dispersed system consisting of a dispersed phase (cell) and a dispersion medium. Microalgae cells are a dispersed phase and are classified according to the degree of particle fragmentation as thin suspensions with particle sizes of 0.5–100 μm. Mos microalgae species have sizes that fit into this range. From a physical point of view, it is impossible to directly measure the absorption of light by culture, but in practice this value is estimated by means of quantitative calculations based on the data obtained by measuring transmittance. In this case, the Bouger-Lambert-Behr law is most often used for calculations. However, the limitations based on the assumptions inherent in this law for turbidity limit its scope for microalga culture suspensions. This is especially true of the generally accepted concept of the optical density of microalgae culture, which, in the strict sense, is not applicable to cell suspensions. In practice, spectrophotometers are most often used to assess the culture spectral properties, and of their many designs, two-beam devices with an integrating sphere are the most suitable for these purposes. Such devices allow considering light scattering, but do not take into account additional light absorption in the pericellular environment, which can significantly contribute to the transmission spectrum. To compensate for this absorption, it is proposed to record the post-centrifugation supernatant transmission spectrum of the culture in parallel. The difference in spectral measurements makes it possible to estimate the spectrum and coefficient of light absorption by photosynthetic cell pigments.

Keywords: microalgae, culture, transmittance spectra, light absorption coefficient

Full text in PDF(RUS)

REFERENCES

  1. Hajlov K.M., Lanskaja L.A., Finenko Z.Z., Ten V.S., Akinina D.K., Sergeeva L.M., Berseneva G.P., Burlakova Z.P., Kondrat’eva T.M., Pavlova E.V., and Petipa T.S. Jekologicheskaja fiziologija morskih planktonnyh vodoroslej (v uslovijah kul’tur) (Ecological physiology of marine planktonic algae (in culture conditions)), Kiev: Nauk. dumka, 1971, 205 p.
  2. Stukolova I.V. and Trenkenshu R.P. Osnovnye tipy pitanija vodoroslej (kratkij glossarij) (The main types of algae nutrition (short glossary)). Voprosy sovremennoj al’gologii, 2020, No. 1 (22), pp. 34–38. http://algology.ru/1588
  3. Trenkenshu R.P., Borovkov A.B., and Lelekov A.S. Ustanovka dlja kul’tivirovanija nizshih fototrofov (Plant for cultivation of lower phototrophs). Patent RU 150345 U1. MPK C12N 1/12. Opubl. 2015‑02-10. Byul. No. 4.
  4. Voskresenskaja N.P. Fotosintez i spektral’nyj sostav sveta (Photosynthesis and spectral composition of light), Moscow: Nauka, 1965, 309 p.
  5. Paramonov L.E. Metod T-matric i kvantovaja teorija uglovogo momenta v zadachah rassejanija i pogloshhenija sveta ansambljami chastic nesfericheskoj formy: dis. … d-ra fiz.-mat. nauk (The T-matrix method and quantum theory of angular momentum for problems of light scattering and absorption by non-spherical particle ensembles. Dr. phys.-math. sci. thesis), Tomsk: IOA SO RAN, 1995, 230 p.
  6. Paramonov L.E. Ocenka soderzhanija hlorofilla po spektram pogloshhenija nativnyh kletok Spirulina platensis (Estimation of chlorophyll content by absorption spectra of native Spirulina platensis cells). Voprosy sovremennoj al’gologii, 2020, No. 1 (22), pp. 25–33. http://algology.ru/1607
  7. Coglin L.N. Cikly razvitija kletok i fiziologicheskie svojstva popu-ljacij mikrovodoroslej: avtoref. dis. … d-ra biol. nauk (Cell development cycles and physiological properties of microalgal populations. Synopsis of Dr. biol. sci. thesis), Moscow: IFR, 1996, 77 p.
  8. Finenko Z.Z. Jekologo- fiziologicheskie osnovy pervichnoj produkcii v more: avtoref. dis. … d-ra biol. nauk: (Ecological and physiological basis of primary production in the sea. Synopsis of Dr. biol. sci. thesis), Sevastopol, InBJuM, 1976, 46 p.
  9. Tejlor D., Grin N., and Staut U. Biologija. Tom 1 (Biology. Vol. 1), Moscow: Laboratorija znanij, 2021, 454 p.
  10. Zhondareva Ja.D. and Trenkenshu R.P. Miksotrofnyj rost Tetraselmis viridis v aksenicheskoj nakopitel’noj kul’ture (Mixotrophic growth of Tetraselmis viridis in axenic culture). Voprosy sovremennoj al’gologii, 2024, No. 1 (24), pp. 21–28. http://algology.ru/2097

Loading