Monitoring of the cyanobacteria composition in bottom sediments of the water area of the Black and Azov seas along the coast of Crimea

by

N.A. Andreeva

Institute of Natural and Technical Systems, RF, Sevastopol, Lenin St., 28

Е-mail: andreeva.54@list.ru

DOI: 10.33075/2220-5861-2021-1-107-117

UDC 561.232+574.586 (262.5+262.54)    

Abstract:

As a result of laboratory cultivation of bottom sediment samples taken during six expeditions at oceanographic stations of the coastal waters of the Black and Azov Seas located along the coast of Crimea in different seasons of 2019‒2020, preliminary results on the distribution of cyanobacteria in the benthos of these areas were obtained. The development of cultivated forms of cyanobacteria was observed in 65 Black Sea samples at 54 stations and in all samples from the Sea of Azov. In the benthos of the Black and Azov Seas areas, representatives of 11 and 10 genera of cyanobacteria were identified, respectively, belonging to four orders: Synechococcales (Synechococcus, Rhabdoderma, Aphanocapsa, Merismopedia, Leptolyngbya), Chroococcales (Microcystis, Chroococcus), Oscillatoriales (including Spirulina), Nostocales (Anabaena, Nostoc, Scytonema). Representatives of the genus Microcystis were the most common in the bottom sediments of the Black Sea coast of Crimea, whereas in the Sea of Azov they were Microcystis and Oscillatoriales. Cyanobacteria with a deep violet color were often found, which indicates the predominance of phycoerythrin in the pigment complex of these cyanobacteria. The maximum number of cultivated forms of cyanobacteria in the bottom sediments of the Black Sea during the entire study period was registered in the euphotic zone, and the lowest – in the 100–1000 m zone.

40 batch cultures were obtained, from which new strains of cyanobacteria are be isolated.

In the future, the research results can be used to monitor the ecological state of the coastal waters, while the isolated cyanobacteria strains can also be used for genetic, biotechnological, medical and other research.

Keywords: expeditions, the Black Sea, the Azov Sea, bottom sediments, cyanobacteria, batch cultures.

To quote: Andreeva, N.A. “Monitoring of the Cyanobacteria Composition in Bottom Sediments of the Water Area of the Black and Azov Seas Along the Coast of Crimea.” Monitoring Systems of Environment no. 1 (March 25, 2021): 107–117. doi:10.33075/2220-5861-2021-1-107-117.

Full text in PDF(RUS)

LIST OF REFERENCES

  1. Bentosnye vodorosli. 2015. https://studfiles.net/preview/1743728/page:4/ (data obrashhenija: 18.08.2020).
  2. Barinova S.S. Jekologicheskie gruppirovki cianobakterij i makro-klimaticheskie faktory, vlijajushhie na ih rasprostranenie. Cianoprokario-ty/cianobakterii: sistematika, jeko-logija, rasprostranenie: materialy dokladov II Mezhdunar. nauch. shkoly-konf., 16–21 sentjabrja 2019 g., Syk-tyvkar, Rossija. Syktyvkar: IB FIC Komi NC UrO RAN, 2019. pp. 9–12.
  3. Kamnev A.N. Jekologicheskaja fi-ziologija vodnyh fototrofnyh orga-nizmov. Chast’ 1. Vodnye oksigennye fototrofy. Voprosy sovremennoj al’gologii (Issues of modern algology) – algology.ru. 2013. No 1 (3). S. URL: http://algology.ru/93
  4. Vitchenko T.V. Strukturno-produkcionnye harakteristiki mor-skogo mikrofitobentosa litoral’noj zony Vostochnogo Murmana: avtoref. dis. … kand. biol. nauk. M., 2005. 19 p.
  5. Underwood G.J.C. Microphyto-benthos. Encyclopedia of Ocean Sciences (Second Edition), 2001. https://www.sciencedirect.com/topics/earth-and-planetary sciences/microphytobenthos.
  6. Watermann F., Hillebrand H., Gerdes G. et al. Competition between benthic cyanobacteria and diatoms as influenced by different grain sizes and temperatures. Mar Ecol Prog Ser. 1999. Vol. 187. pp. 77–87.
  7. Golubic S., Seong-Joo L., Browne K.M. Cyanobacteria: Architects of Sedi-mentary Structures. In: Microbial Sedi-ments. Springer-Verlag Berlin Heidel-berg, 2000. pp. 57‒67. DOI: https://doi.org/10.1007/978-3-662-04036-2_8
  8. Senatskaja E.V., Averina S.G., Pinevich A.V. Novye predstaviteli cianobakterij s pigmentami, poglo-shhajushhimi dal’nij krasnyj svet. Cianoprokarioty/cianobakterii: siste-matika, jekologija, rasprostranenie: materialy dokl. II Mezhdunar. nauch. shkoly-konf., 16–21 sentjabrja 2019 g., Syktyvkar, Rossija. Syktyvkar: IB FIC Komi NC UrO RAN, 2019. pp. 234–237.
  9. Prakticheskaja gidrobiologija. Presnovodnye jekosistemy / pod red. V.D. Fedorova, V.I. Kapkova. M.: Izd-vo PIM, 2006. 367 p.
  10. Gorin K.K., Beljakova R.N. Predvaritel’nye dannye o bentosnyh Cyanoprokaryota vostochnoj chasti Finskogo zaliva Baltijskogo morja. Cianoprokarioty/cianobakterii: si-stematika, jekologija, rasprostranenie: materialy dokl. II Mezhdunar. nauch. shkoly-konf., 16–21 sentjabrja 2019 g., Syktyvkar, Rossija. Syktyvkar: IB FIC Komi NC UrO RAN, 2019. pp. 112–115.
  11. Rjabushko L.I. Sostojanie izuchennosti mikrofitobentosa Argentinskih ostrovov Antarktiki // Prirodnaja sreda Antarktiki: sovremennoe sostojanie izuchennosti: materialy konf. Trudy BGU. 2016. Vol. 11. Ch. 1. pp. 337–350.
  12. Kolda A., Ljubešić Z., Gavrilović A. et al. Metabarcoding Cyanobacteria in coastal waters and sediment in central and southern Adriatic Sea. Acta Bot. Croat. 2020. Vol. 79 (2). pp. 157–169. DOI: 10.37427/botcro-2020-021
  13. Sooroojebally R., Bhagea R., Nadeem N., Bhoyroo V. Marine cyano-bacteria from Mauritian waters // Phycology International. 2018. Vol. 1. No 57. pp. 5‒9.
  14. Ford A.K., Bejarano S., Nugues M.M. et al. Reefs under Siege ‒ the Rise, Putative Drivers, and Consequences of Benthic Cyanobacterial Mats. Front. Mar. Sci. 2018 | https://doi.org/10.3389/fmars.2018.00018
  15. Sun F.-L., Wang Y.-S., Wu M.-L., Sun C.-C. Cyanobacterial community diversity in the sediments of the Pearl River Estuary in China. Sci. Mar. 2017. Vol. 81 (4). pp. 477–485. DOI: http://dx.doi.org/10.3989/scimar.04106.07A
  16. Bondarenko A.V. Mikrovodorosli bentosa krymskogo pribrezh’ja Azovskogo morja: dis. … kand. biol. nauk. Sevastopol’, 2017. 237 p.
  17. Balycheva D.S., Rjabushko L.I. Mikrovodorosli bentosa zapovednika «Lebjazh’i ostrova» (Chernoe more). Zapovednaja nauka. 2017. Vol. 2 (Suppl. 2). pp. 9–18. DOI: 10.24189/ncr.2017.027
  18. Vinogradova O.N., Brjanceva Ju.V. Taksonomicheskaja revizija Syanobacteria / Cyanoprokaryota cher-nomorskogo poberezh’ja Ukrainy. Algologia. 2017. Vol. 27(4). pp. 436‒457. DOI: 10.15407/alg27.04.436
  19. Rjabushko L.I., Bondarenko A.V., Li R.I. Mikrovodorosli morskih akvatorij goszakaznika «Buhta Kazach’ja», Karadagskogo (Chjornoe more) i Kazantipskogo (Azovskoe more) zapovednikov Ukrainy: materіali HIII z’їzdu Ukraїns’kogo botan. tovaristva L’vіv, 19–23 veresnja 2011 r. L’vіv, 2011. 321 p.
  20. Zaitsev Y.P., Polikarpov G.G. Re-cently Discovered New Biospheric Pelocontour Function in the Black Sea Reductive Bathyal Zone. J. Black Sea / Mediterranean Environment. 2008. Vol. 14. pp. 151–165.
  21. Vasser S.P., Kondrat’eva N.V., Masjuk N.P. i dr. Vodorosli. Spravochnik. Kiev: Naukova dumka, 1989. 608 p.
  22. Kondrat’eva N.V. Sin’ozeleni vodorosti – Cyanophyta. Ch. 2. Klass Gormogoniєvi – Hormogoniophyceae: v kn. Viznachnik prisnovodnih vodoro-stej Ukraïns’koï RSR. Vol. 1. Kiïv: Naukova dumka, 1968. 523 p.
  23. Kondrat’eva N.V., Kovalenko O.V., Prihod’kova L.P. Sin’ozeleni vodorosti – Cyanophyta. Zagal’na ha-rakteristika sin’ozelenih vodoro-stej – Cyanophyta. Klass Hrookokovi – Chroococcophyta. Klass Hamesifonovi – Chamaesiphonophyceae: v kn. Viznachnik prisnovodnih vodorostej Ukraïns’koï RSR. Vol. 1. Kiïv: Naukova dumka, 1984. 388 p.
  24. Komárek J., Kaštovsky J., Mareš J., Johansen J.R. Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. Preslia. 2014. No 86. pp. 295–335.
  25. Stomp M., Huisman J., de Jong F., et al. Adaptive divergence in pigment composition promotes phytoplankton biodiversity. Nature. 2004. Vol. 432. pp. 104–107.
  26. Impacts of Climate Change on the Occurrence of Harmful Algal Bloom // United States Environmental Protection Agency. 2013 /https://www.epa.gov/sites/production/files/documents/climatehabs.pdf (data obrashhenija: 19.10.2020).

Loading