I.S. Khalikov1, V.I. Baranov2
1 Federal State Budgetary Institution “Research and Production Association “Typhoon” (“RPA
“Typhoon”), Russian Federation, Obninsk, Pobedy St., 4
2 South Branch of P.P.Shirshov Institute of Oceanology, Russian Academy of Sciences, Russian
Federation, Gelendzhik, Prostornaya St., 1-g
Optimization of phenol detection by method of high performance liquid chromatography (HPLC) with the use of a fluorometric detector has been carried out. To effectively extract phenol from bottom sediments a method for sample preparation with the use of 25% acetonitrile under the action of ultrasound has been worked out. The limit of phenol extraction made 90–100% with addition of phenol from 10 ng/g to 2000 ng/g. The degree of phenol detection (the ratio signal/noise = 3) in the samples of bottom sediments made about 1 ng/g. To assess the applicability of the method proposed for phenol determination, 122 samples of bottom sediments from the Arctic seas were analyzed. Average phenol content in the regions of the Barents sea studied (12,4 ng/g, n = 52) was 1,5 times higher than that in the Kara sea (8,2 ng/g, n = 70).
Keywords: phenol, fluorometric detector, HPLC, bottom sediments, Barents and Kara seas.
LIST OF REFERENCES
- Glukhareva E. K. Prospects for production and transportation of oil and gas resources in the West of the Russian Arctic // Problems of forecasting. 2011. No. 5. P. 65-75.
- Lurie Yu. Y. Analytical chemistry of industrial wastewater. M.: Chemistry, 1984. P. 447.
- Kondratieva L. M., Garetova L. A., Chukhlebova L. M. Microbiological assessment of environmental risk of transboundary pollution of the Amur river. Vladivostok: Dalnauka, 2002. P. 198.
- Dauvalter V. A. Geoecology of bottom sediments of lakes. Murmansk: MSTU Publishing house, 2012. P. 242.
- Strakhov N. M. Selected works. General problems of Geology, lithology and Geochemistry, Moscow: Nauka, 1983, P. 640.
- Baker M.D., Mayfield C.I. Microbial and nonbiological decomposition of chlorophenols and phenol in soil // Water Air Soil Pollut. 1980. Vol. 13. P. 411–424.
- Scott H.D., Wolf D.C., Lavy T.L. Apparent absorption and microbial degradation of phenol by soil // J. Environ. Qual. 1982. Vol. 11. P. 107–112.
- Fedorak P.M., Hrudey S.E. Nutrient requirements for the methanogenic degradation of phenol and p-cresol in anaerobic draw and feed cultures // Water Res. 1986. Vol. 20. P. 929–934.
- Mineralization potential for phenol in subsurface soils / D.C. Dobbins, J.R. Thornton-Manning, D.D. Jones [et al] // J. Environ. Qual. 1987. Vol.16. P. 54–58.
- Instrumental methods for the analysis of functional groups of organic compounds / TRANS. from English. ed. by S. Siggia. M.: Mir, 1974. P. 464.
- Vorobyova T. V., Terletskaya A.V., Kushchevskaya N. F. Standard and unified methods for determining phenols in natural and drinking waters and the main directions of their improvement. 2007. Vol. 29, No. 4. P. 370-390.
- Sursyakova V. V., Burmakina G. V., Rubailo A. I. Development of methods for determining phenols in drinking and natural waters by capillary electrophoresis and high-performance liquid chromatography / / Journal of the Siberian Federal University. Series: Chemistry. 2010. Vol. 3, No. 3. P. 268-277.
- Korenman Ya. I., Gruzdev I. V., Kondratenok B. M. Gas chromatographic determination of chlorophenols in drinking water with preliminary two-stage chemical modification. 2000. Vol. 22, No. 3. P. 290-297.
- Vermeulen А., Welvaert K., Vercammen J. Evaluation of a dedicated gas chromatography-mass spectrometry method for the analysis of phenols in water // Journal of Chromatography A. 2005. Vol. 1071, № 1–2. Р. 41–46.
- Zhang P.-P, Shi Z.-G., Feng Y.-Q. Determination of phenols in environmental water samples by two-step liquid-phase microextraction coupled with high performance liquid chromatography // Talanta. 2011. Vol. 85. P. 2581–2586.
- Dean J.R., Santamaria-Rekondo A., Ludkin E. Accelerated solvent extraction of phenols from soil // Analytical Communications. 1996. Vol. 33. P. 413–416.
- Extraction and preconcentration techniques for chromatographic determination of chlorophenols in environmental and food samples / P. Morais, T. Stoichev, M. Clara [et al] // Talanta. 2012. Vol. 89. P. 1–11.
- Comparison of microwave-assisted extraction and Soxhlet extraction for phenols in soil samples using experimental designs / A. Egizabal, O. Zuloaga, N. Etxebarria [et al] // Analyst. 1998. Vol.123. P. 1679–1684.
- An organic solvent-free microwave-assisted extraction some priority pollutants of phenols in lake sediments / L. Wang, W. Huang, X. Shao [et al] // Analytical sciences. 2003. Vol. 19. P. 1487–1490.
- Fast and easy multiresidue method employing acetonitrile extraction / partitioning and «dispersive solid-phase extraction» for the determination of pesticide residues in produce / M. Anastassiades, S.J. Lehotay, D. Stajnbaher [et al] // J. AOAC Int.. 2003. Vol. 86. No.2 . P. 412–431.
- Khalikov I. S. Quechers sample Preparation for determining pollutants in natural environment objects // Bulletin of scientific conferences. 2016. no. 5-2(9). P. 111-112.
- Application of a quick, easy, cheap, effective, rugged and safe-based method for the simultaneous extraction of chlorophenols, alkylphenols, nitrophenols and cresols in agricultural soils, analyzed by using gas chromatography–triple quadrupole-mass spectrometry/mass spectrometry / J.A. Padilla-Sánchez, P. Plaza-Bolaños, R. Romero-González [et al] // Journal of Chromatography A. 2010. Vol. 1217, Р. 5724–5731.
- Multiresidue analysis of aromatic organochlorines in soil by gas chromatography-mass spectrometry and QuEChERS extraction based on water/dichloromethane partitioning. Comparison with accelerated solvent extraction / F. Rouviere, A. Bulete, C. Cren-Olive [et al] // Talanta. 2012. Vol.93. P. 336–344.
- Kola maps 2006-2012. Overview map of the Barents and Kara seas-URL: http://www.kolamap.ru/arh/shtokman.html (date of request: 18.04.2018).