Structure of mechanoreception and adhesion capability in juvenile mussels mytilus galloprovincialis (lamarck, 1819) under ultrasound impact

by

A.V. Kuznetsov1,2, A.N. Petrov1, A.V. Pirkova1, E.V. Sergeeva2

1The A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS,

RF, Sevastopol, Nakhimov Av., 2

  E-mail:  kuznet61@gmail.com, alexpet-14@mail.ru

2Sevastopol State University, RF, Sevastopol, Universitetskaya St., 33

DOI: 10.33075/2220-5861-2023-4-41-51

UDC 594.124:[57.043:534.321.9]

EDN: https://elibrary.ru/speyxa

Abstract:                             

The experiments on the effect of ultrasound on the adhesive ability of juvenile mussel Mytilus galloprovincialis Lam. Are carried out. It is revealed that the acoustic cavitation induced by ultrasound with a power of 50 W and frequency of 42 kHz stimulates animal group activity, resulting in the formation of mussel druses and strong cohesion on the surface of experimental vessels. On the contrary, the mussels detach from the surface after extended sound exposure within the frequency range of 1–30 kHz, and the proportion of detached mollusks increases depending on the duration of the experiments.

The mussel contains a family of five TRP-channels (VDH91361.1, VDI64650.1, VDI64648.1, VDI64649.1 and VDI13976.1) with individual subunit chain lengths ranging from 1157 to 1349 aa, as well as one Piezo-channel, VDI27946.1, with a length of 2377 aa. Spatial models of VDI13976.1 and VDI27946.1 proteins are constructed; the potential role of TRP- and Piezo-channels as mechanoreceptors in adhesion regulation is considered.

The results obtained can be used both for optimizing the regimes of ultrasonic treatment of surfaces in water conduits and technical water supply marine systems in order to prevent their undesirable colonization by periphyton organisms and for studying in more details the structure of mechanoreceptors as well as  the role of TRP and Piezo channels in adhesion regulation in mussels.

Keywords: Mytilus galloprovincialis, ultrasound, adhesion, mechanoreceptors, TRP-channels, Piezo-channels.

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