Сomparative assessment of the chlorophyll “a” concentration in the black sea in the spring according to the data of the sounding complex and direct measurements

by

A.A. Farber, L.V. Stelmakh

The A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS,

RF, Sevastopol, Nakhimov Av., 2

DOI: 10.33075/2220-5861-2024-1-53-61

UDC 582.261.1(262.5)                                                

EDN: https://elibrary.ru/fswzfn

Abstract:

As an express method for determining the content of chlorophyll “a” in the phytoplankton of the Black Sea, in vivo fluorescence is often used, determined using a submersible fluorescent probe directly during outboard work. In this paper, a comparative assessment of determination of the concentration of this pigment is carried out with the data obtained using a fluorescent probe and direct measurements in March-April when the water temperature stratification does not occur. For the stations located in the shelf area (< 200 m) and the continental slope (200–1500 m), the relationship between chlorophyll content measured in acetone extracts and its concentration calculated from in vivo fluorescence is described by linear equations with an approximation confidence of 72–89%. However, the values of the equation coefficients show that the content of this pigment, according to the probe data, is 1.69–2.89 times higher compared to the results of direct measurements. Based on literature analysis, the reasons for the obtained differences related to the variability of some algae cell parameters reflecting their functioning are discussed. Among such parameters, the average value of the specific indicator of light absorption by phytoplankton pigments, the fluorescent intracellular reabsorption factor and the quantum yield of fluorescence in vivo, the variability of which depends on both environmental conditions and phytoplankton species and taxonomic composition must be highlighted. It can be concluded that in vivo chlorophyll “a” fluorescence data should only be used to assess the nature of its vertical distribution in the sea.

Keywords: phytoplankton, chlorophyll “a”, fluorescence, Black Sea.

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REFERENCES

  1. Behrenfeld, M.J., Boss, E., Siegel, D.A., and Shea, D.M. Carbon‐based ocean productivity and phytoplankton physiology from space. Global biogeochemical cycles, 2005, Vol. 19, GB 1006. https://doi.org/10.1029/2004GB002299
  2. Kovaleva I.V., Finenko Z.Z., and Suslin V.V. Prostranstvennaja i vremennaja dinamika biomassy fitoplanktona v poverhnostnom sloe Chjornogo morja. (Spatial and temporal dynamics of the phytoplankton biomass in the surface layer of the Black Sea). Morskoj biologicheskij zhurnal, 2023, Vol. 8, No. 4, pp. 52–63. https://doi.org/10.21072/mbj.2023.08.4.04
  3. Kovalyova I.V. and Suslin V.V. Integrated Primary Production in the Deep-Sea Regions of the Black Sea in 1998–2015. Physical Oceanography, 2022, Vol. 29, No. 4, pp. 404–416. https://doi.org/10.22449/1573-160X-2022-4-404-416
  4. Stelmakh L. and  Kovrigina N. Phytoplankton Growth Rate and Microzooplankton Grazing under Conditions of Climatic Changes and Anthropogenic Pollution in the Coastal Waters of the Black Sea (Sevastopol Region). Water, 2021, Vol. 13, Issue 22, Article No. 3230, 13 p. https://doi.org/10.3390/w13223230
  5. Finenko Z.Z., Churilova T.Ja., and Li R.I. Vertikal’noe raspredelenie hlorofilla i fluorescencii v Chjornom more (Vertical distribution of chlorophyll and fluorescence in the Black Sea). Morskoj jekologicheskij zhurnal, 2005, Vol. 4, No. 1, pp. 15–46.
  6. Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS Report Nr. 19, vi+170 pp. Reprint of the IOC Manuals and Guides No. 29. UNESCO 1994.
  7. Lorenzen C.J. A method for the continuous measurement of in vivo chlorophyll concentration. Deep Sea Research and Oceanographic Abstracts, 1966, Vol. 13, No. 2, pp. 223–227. https://doi.org/10.1016/0011-7471(66)91102-8
  8. Krasheninnikova S.B. and Babich S.A. Prostranstvennoe raspredelenie koncentracii hlorofilla “a” s uchjotom gidrologicheskih, gidrohimicheskih i gidroopticheskih uslovij Chjornogo morja vesnoj 2021 g. (Spatial distribution of chlorophyll a concentration in hydrological, hydrochemical and hydroptical condi-tions of the Black Sea in spring 2021). Trudy Karadagskoj nauchnoj stancii im. T.I. Vjazemskogo – pri-rodnogo zapovednika RAN, 2022, Vol. 7, No. 3 (23), pp. 13–22.
  9. Mansurova I.M., Stel’mah L.V., and Farber A.A.Vertikal’noe raspredelenie koncentracii hlorofilla “a” v Chernom more v letnij i osennij periody po dannym zondirujushhego kompleksa i prjamyh izmerenij // Sistemy kontrolja okruzhajushhej sredy. (Vertical distribution of chlorophyll a concentration in the Black Sea in summer and autumn according to the data of the sounding complex and direct measurements). Sistemy kontrolja okruzhajushhej sredy, 2023, No. 2 (52), pp. 84–91. https://doi.org/10.33075/2220-5861-2023-2-84-91
  10. Finenko Z.Z., Mansurova I.M., Kovaljova I.V., and Georgieva E.Ju. Razvitie fitoplanktona v zimne-vesennij period v pribrezhnyh vodah Kryma (Development of phytoplankton in the winter-spring period in the coastal waters of Crimea). Morskoj biologicheskij zhurnal, 2021, Vol. 6, No. 1, pp. 102–114. https://doi.org/10.21072/mbj.2021.06.1.08
  11. 11. Moiseeva N.A., Churilova T.Ja., Efimova T.V., Krivenko O.V., and Matorin D.N. Koncentracija i fluorescencija hlorofilla “a” v period sezonnoj stratifikacii vod v Chernom more (Fluorescence of Chloro-phyll a During Seasonal Water Stratification in the Black Sea). Morskoj gidrofizicheskij zhurnal, 2019, Vol. 35, No. 5, pp. 481–495. https://doi.org/10.22449/0233-7584-2019-5-481-495
  12. Moiseeva N.A., Churilova T.Ja., Efimova T.V., Artem’ev V.A., and Skorohod E.Ju. Spektral’nye bioopticheskie pokazateli vod Atlanticheskogo sektora Antarktiki (Spectral bio-optical properties of water of Atlantic sector of Antarctic). Morskoj biologicheskij zhurnal, 2020, Vol. 5, No. 4, pp. 69–78. https://doi.org/10.21072/mbj.2020.05.4.06
  13. Vedernikov V.I. Pervichnaja produkcija i hlorofill v Chernom more v letne-osennij period / Struktura i produkcionnye harakteristiki planktonnyh soobshhestv Chernogo morja (Primary production and chlorophyll in the Black Sea in the summer-autumn period). (Structure and production characteristics of plankton communities in the Black Sea), Moscow: Nauka, 1989, pp. 65–83.
  14. Kubrjakov A.A. Belokopytov V.N., Zacepin A.G., Stanichnyj S V., and Piotuh V.B. Izmenchivost’ tolshhiny peremeshannogo sloja v Chernom more i ee svjaz’ s dinamikoj vod i atmosfernym vozdejstviem (The Black Sea mixed layer depth variability and its relation to the basin dynamics and atmospheric forcing). Morskoj gidrofizicheskij zhurnal, 2019, Vol. 35, No. 5 (209), pp. 449–468. https://doi.org/10.22449/0233-7584-2019-5-449-468
  15. Vinberg G.G., Muravleva E.P., and Finenko Z.Z. Nekotorye dannye po soderzhaniju hlorofilla i pervichnoj produkcii v planktone Chernogo morja (Some data on the content of chlorophyll and primary production in plankton of the Black Sea). Tr. Sevastop. biol. Stancii, 1964, Vol. 17, pp. 212–220.
  16. Babin M. Phytoplankton fluorescence: theory, current literature and in situ measurement. (Realtime Coastal Observing Systems for Marine Ecosystem Dynamics and Harmful Algal Blooms: Theory, Instrumentation and Modelling), Paris: UNESCO Publishing, 2008, pp. 237–280. https://doi.org/10.25607/OBP-1369
  17. Bricaud A., Babin M., Morel A., and Claustre H. Variability in the chlorophyll‐specific absorption coefficients of natural phytoplankton: Analysis and parameterization. Journal of Geophysical Research: Oceans, 1995, Vol. 100, No. C7, pp. 13321–13332. https://doi.org/10.25607/OBP-1369
  18. Morel A. and Bricaud А. Theoretical results concerning light absorption in a discrete medium, and application to specific absorption of phytoplankton., Deep-Sea Research Part A: Oceanographic Research Papers, 1981, Vol. 28, No. 11, pp. 1375–1395. https://doi.org/10.1016/0198-0149(81)90039-X
  19. Falkowski P. and Kiefer D.A. Chlorophyll a fluorescence in phytoplankton: relationship to photosynthesis and biomass. Journal of Plankton Research, 1985, Vol. 7, No. 5, pp. 715–731. https://doi.org/10.1093/plankt/7.5.715
  20. Babin M., Morel A., Claustre H., Bricaud A., Kolber Z., and Falkowski P.G. Nitrogen and irradiance-dependent variations of the maximum quantum yield of carbon fixation in eutrophic, mesotrophic and oligotrophic marine systems. Deep Sea Research Part I: Oceanographic Research Papers, 1996, No. 43(8), pp. 1241–1272. https://doi.org/10.1016/0967-0637(96)00058-1

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