The use of experimental laboratory stand for the study of optical properties of the aquatic medium in the presence of microbiota

 S.A. Sholar1, O.A. Stepanova2, L.V. Stelmach3

 1Marine Hydrophysical Institute of RAS, RF, Sevastopol, Kapitanskaya St., 2

E-mail: sa.sholar@mail.ru

2Institute of Natural and Technical Systems, RF, Sevastopol, Lenin St., 28

Email: solarua@ya.ru

3The A.O. Kovalevsky Institute of Marine Biological Research of RAS, RF, Sevastopol, Nachimov Av., 2

E-mail: lustelm@mail.ru

DOI: 10.33075/2220-5861-2019-2-13-21

UDC 535.8; 581.526.325.3; 578.4

Abstract:

    As a part of experiments to study optical properties of the aquatic environment in the presence of microbiota a simple and easy-to-use experimental laboratory stand was adapted and tested. The stand is a construction of two identical transparent tanks of equal volume (up to 15 l), intended for control and experimental measurements of the optical characteristics of sea water using a small-size multi-spectral light beam attenuation meter under the conditions of the same illumination and temperature.

   In tanks, model biological systems are created, the basis of which is pasteurized seawater, to which microbiota is added, which affects the optical properties of the aqueous medium (environment). As microbiota either cultures of certain types of microalgae or mixed cultures are used. At the same time, viral suspension of algal virus is added to the experiment (test), and an equal volume of pasteurized sea water is added to the control.

   The experiments performed with measuring the attenuation of light beam attenuation coefficient in model biological systems revealed the effect of microbiota on the optical properties of the aquatic environment: an increase in the attenuation of light as a result of the growth of microalgae cultures (in control) and its decrease due to viral culture lysis (in the experiment).

Keywords: laboratory stand, small-size multi-spectral light beam attenuation meter, microalgae culture, algal viruses, viral lysis.

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