Automation possibilities of search for viruses in aquatic environment

by

S.A. Sholar1,2, O.A. Stepanova2

 1FRC Marine Hydrophysical Institute of RAS, RF, Sevastopol, Kapitanskaya St., 2

 E-mail: sa.sholar@mail.ru

2Institute of Natural and Technical Systems, RF, Sevastopol, Lenin St., 28

 E-mail: solar-ua@ya.ru

DOI: 10.33075/2220-5861-2023-3-50-57

UDC 577.35. 578.4. 57.08. (262.5)                                                                                  

Abstract:

Currently, the role of viruses in possible epidemics is increasing, so the danger of biological threats (danger) using viruses as biological weapons is also increasing. Taking into account the increasing importance of viruses as pathogenic objects, as well as against the background of the rapid development of marine virology, there is a need to create simple and accessible, including automated, methods for determining (indication and identification) the viruses, some algal viruses as well, and their concentration directly in the studied water areas to obtain an express assessment of the ecological situation.

The purpose of the paper is a brief review and analysis of the available literature data on the application of automation in biological studies of micro and nano bio-objects, and devices, installations and setups created for this.

A brief review of the existing express methods for the indication and identification of viruses, with algal viruses of the Black Sea included, in laboratory and field material, including hydrosphere water, is made. It is established that the devices and methods described in the literature and among patents, are based on molecular-genetic, immune and immunochemical, electrical, biosensor, photo-graphic and other properties and reactions. The advantages and disadvantages of the described methods and installations are analyzed. Proposals for automating some of these methods – the reaction of coagglutination and polymerase chain reaction, are substantiated.

Keywords: express methods for indication and identification of viruses, marine viruses, Black Sea algal viruses, coagglutination reaction, polymerase chain reaction (PCR).

To quote: 

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