Improving the selectivity of the biosensor early warning system exposed to acoustic vibrations

by

A.N. Grekov, N.A. Grekov, K.A. Kuzmin, S.S. Peliushenko

 Institute of Natural and Technical Systems, RF, Sevastopol, Lenin St., 28

E-mail: i@angrekov.ru

DOI: 10.33075/2220-5861-2023-3-68-78 

UDC 57.084                                                                                     

Abstract:

The paper presents the results of a study and assessment of the impact of acoustic and vibration signals on Black Sea bivalve mussels, which made it possible to determine the technical characteristics of acoustic vibration sensors required for installation in an automated marine early warning biosystem and use the developed methodology in signal processing to increase the selectivity of the response to pollutants.

The developed method is described, which consists in the fact that after exposing a colony of mussels to various stimuli, the time of simultaneous closing of the valves of all mussels, recorded by the valve motion sensor in the form of a monotonically decreasing function, was analyzed, or rather the value of the time interval, which was determined between the beginning of the movement of the mussel valves and the stop of the movement or their complete closure, which made it possible to eliminate the calibration of the opening value of the mussel valves at the stage of manufacturing and setting up the biosensor system, as well as to control and determine false positives or incomplete opening of the valves of individual mussels when exposed to point stimuli.

Bioindication, being simple, allows one to obtain information about biological changes in the environment and draw indirect conclusions about the characteristics of the factor itself. Thus, when assessing the state of the environment, it is desirable to selectively supplement the biological system with channels that measure physicochemical parameters and channels to eliminate false alarms, which will allow one to obtain qualitative and quantitative characteristics of the marine environment. A structural and functional diagram of the developed experimental setup is presented, its composition is listed, and the operation is described.

Keywords: control, frequencies, response of mussel valves, pollution, signal processing, method, calibration, histogram, accelerometer.

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